Journal: Cancer science

Article Title: Dietary supplementation with branched-chain amino acids suppresses diethylnitrosamine-induced liver tumorigenesis in obese and diabetic C57BL/KsJ-db/db mice.

doi: 10.1111/j.1349-7006.2009.01402.x

Figure Lengend Snippet: Fig. 8. Effect of branched-chain amino acid (BCAA) supplementation on hepatic cell proliferation in diethylnitrosamine-treated C57BL ⁄ KsJ- db ⁄ db mice. (a) Immunohistochemical expression of proliferating cell nuclear antigen (PCNA) in the liver of basal diet (CRF-1)-fed, or casein- or BCAA-supplemented treated mice. (b) PCNA-labeling index in non- lesional hepatocytes was determined by counting the PCNA-positive nuclei in the hepatocytes. *P < 0.05; **P < 0.01.

Article Snippet: Immunohistochemistry of a-SMA, an indicator of HSC activation, was carried out using a primary anti-a-SMA antibody (Dako, Glostrup, Denmark). (22) Immunohistochemistry of PCNA, a G1-to-S phase marker, was carried out to estimate the cell proliferative activity of the hepatocyte using a primary anti-PCNA antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA). (23) PCNA-positive nuclei in the hepatocytes were counted and expressed as the percentage of the total number of hepatocyte nuclei.

Techniques: Immunohistochemical staining, Expressing, Labeling